FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9785798
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC179930.18386850004465655TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT32750.031.4887642
ACGTCTG55750.030.10279515
CGTCTGA57300.029.13145616
TCGTATG34650.027.87831743
CACACGT62650.027.14648412
CACGTCT62250.027.14022614
CAGTCAC61450.026.61493527
ATCGGAA64550.026.525762
TCGGAAG66200.025.9312733
AGTCACC65500.025.2783928
ACACGTC68000.025.0437813
CGTATGC37050.024.4315144
CCAGTCA70350.023.53567126
CGGAAGA73500.023.3251724
GATCGGA74500.023.2573641
TCTCGTA46600.023.23988241
ACTCCAG73650.022.69528423
TCCAGTC73900.022.43550925
AGCACAC76800.022.37922510
GCACACG79050.021.82761811
Produced by FastQC (version 0.11.5)