FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25741090
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324490.1260591528952348No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC277300.10772659588230335TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG67400.029.5384828
ACGTCTG73000.028.01210815
CTCGTAT56300.027.15692342
CGTCTGA76550.026.74243716
GTCACGC78450.025.6072729
TCGTATG56650.024.96294243
TATCTCG74600.023.44440839
CACGTCT88300.023.3621814
ATCGGAA90700.023.0448972
TCGGAAG91300.022.9421163
CACACGT90900.022.91668912
CGCCAAT90150.021.83469433
CAGTCAC93750.021.76410327
ACGCCAA91400.021.53607732
CGTATGC64400.021.43457644
CACGCCA93900.021.17831631
TCACGCC99050.020.80394730
ACACGTC105300.019.95369113
GCCAATA104900.018.76637534
GATCGGA115350.018.2982251
Produced by FastQC (version 0.11.5)