FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22585236
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC254430.11265323948795576TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52550.026.37031215
CTCGTAT47350.026.05938742
TCGTATG49200.025.21753343
CGTCTGA54750.024.94088616
CACACGT67500.021.22965612
TCGGAAG67550.021.1168383
ATCGGAA67800.021.0063022
CGTATGC58950.020.97039244
CACGTCT69050.020.49246814
GTCACAC67350.020.3750929
CAGTCAC74500.018.66119627
AGTCACA77650.017.81726328
TCTCGTA74300.017.12143341
AGTGATC78200.016.88859236
ACACGTC86000.016.87207813
ACACAGT84200.016.48467432
CACAGTG83850.016.3657933
GATCGGA90900.015.8432951
GTATGCC80850.015.76356545
CGGAAGA96450.015.3493024
Produced by FastQC (version 0.11.5)