FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23522706
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41600.021.63053715
CGTCTGA44950.020.0685216
CTCGTAT28100.017.87058842
AGTCACT49650.017.35322628
TCGGAAG57950.015.8016213
ATCGGAA57850.015.5180322
CACACGT58850.015.32846212
CACGTCT60000.015.18463714
CAGTCAC58050.015.07467827
TCGTATG33800.014.657351543
ACTGACC59750.014.26927732
ACACGTC75400.012.6799713
CGTATGC38450.012.18201944
GTCACTG74700.011.89534929
AATCTCG61800.011.69147539
CACTGAC78000.011.24787931
CGGAAGA86050.011.2167514
GATCGGA83650.010.8679851
AGCACAC89250.010.863510
TATAGGC43050.010.60775727
Produced by FastQC (version 0.11.5)