FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24491133
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC971370.3966210954797395TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG192500.039.6164915
ACTTAGG189750.039.25404732
CGTCTGA194750.039.17034516
CTCGTAT135500.037.92085342
TCACTTA198800.037.43313230
ATCGGAA207100.037.307752
TCGTATG131800.037.2263743
AGTCACT200850.037.1406728
TTAGGCA200800.037.08610534
GTCACTT202250.037.02817529
CACTTAG202800.036.861231
TCGGAAG210050.036.7611663
CACGTCT209300.036.6407914
CACACGT213800.035.8906312
CTTAGGC208700.035.8624633
CAGTCAC215550.034.78518727
CGTATGC139550.034.30394744
ACACGTC227300.033.9668213
TAGGCAT221050.033.43515835
GATCGGA232250.033.3298261
Produced by FastQC (version 0.11.5)