FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23535767
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46750.025.21476215
CGTCTGA49050.023.98655316
CTCGTAT34850.022.42103242
TCGTATG34900.021.16354443
TCGGAAG59450.020.3607923
ATCGGAA58800.020.1656952
CACGTCT61250.019.57610514
CACCGAT58950.019.3095431
GATGTAT59250.019.25153735
CACACGT65300.018.5686712
ACCGATG62900.018.09693732
CGTATGC42250.017.58854744
CAGTCAC67900.017.32754727
TATCTCG57600.016.6470739
AGTCACC74100.016.02953528
ACACGTC80400.015.1652113
GATCGGA81700.014.5979511
GTATCTC77350.014.54654138
CGGAAGA89050.014.1487584
TGTATCT79700.014.0584437
Produced by FastQC (version 0.11.5)