FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l01_n01_P2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l01_n01_P2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23137863
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC348190.1504849432291997TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG74000.031.82909615
CGTCTGA74250.031.66133116
TCGTATG60100.029.78510743
CTCGTAT58400.029.61054642
TCGGAAG87200.027.3750673
ATCGGAA87900.026.978242
CACGTCT88650.026.6706314
CGTATGC65950.026.39204644
CACACGT90550.026.08616312
CAGTCAC93950.025.07028227
TCACGAT91400.024.83717535
GTCACAT100600.023.14470729
AGTCACA102450.022.70481328
ACACGTC107150.022.61168113
ATCACGA104500.022.08943234
CATCACG108150.021.46665633
GATCGGA112350.021.3501741
CCAGTCA110600.021.17409926
CGGAAGA119200.020.4224224
AGCACAC119700.020.33491910
Produced by FastQC (version 0.11.5)