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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-05-05, 05:05 based on data in: /beegfs/mk5636/logs/html/CCLM1ACXX/8

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CCLM1ACXX_l08_n01_S3W1
        28.1%
        47%
        0.0
        CCLM1ACXX_l08_n01_S3W2
        70.1%
        52%
        40.1
        CCLM1ACXX_l08_n01_S3W3
        63.3%
        51%
        32.4
        CCLM1ACXX_l08_n01_S3W4
        69.6%
        52%
        43.4
        CCLM1ACXX_l08_n01_S3W5
        68.0%
        52%
        29.6
        CCLM1ACXX_l08_n01_S3W6
        63.8%
        51%
        30.9
        CCLM1ACXX_l08_n01_S3W7
        64.8%
        51%
        32.8
        CCLM1ACXX_l08_n01_S3W8
        67.2%
        52%
        30.9
        CCLM1ACXX_l08_n01_S3W9
        67.4%
        52%
        32.0
        CCLM1ACXX_l08_n01_undetermined
        91.2%
        45%
        13.5
        CCLM1ACXX_l08_n02_S3W1
        25.4%
        48%
        0.0
        CCLM1ACXX_l08_n02_S3W2
        68.3%
        55%
        40.1
        CCLM1ACXX_l08_n02_S3W3
        63.0%
        53%
        32.4
        CCLM1ACXX_l08_n02_S3W4
        68.5%
        54%
        43.4
        CCLM1ACXX_l08_n02_S3W5
        65.6%
        55%
        29.6
        CCLM1ACXX_l08_n02_S3W6
        62.1%
        53%
        30.9
        CCLM1ACXX_l08_n02_S3W7
        63.5%
        54%
        32.8
        CCLM1ACXX_l08_n02_S3W8
        65.4%
        55%
        30.9
        CCLM1ACXX_l08_n02_S3W9
        66.0%
        55%
        32.0
        CCLM1ACXX_l08_n02_undetermined
        88.6%
        45%
        13.5

        Lane 8 Demultiplexing Report

        Lane 8 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        13,515,751
        4.7
        S3W1
        34,146
        0.0
        S3W2
        40,067,095
        14.0
        S3W3
        32,386,272
        11.3
        S3W4
        43,398,487
        15.2
        S3W5
        29,606,654
        10.4
        S3W6
        30,897,043
        10.8
        S3W7
        32,804,928
        11.5
        S3W8
        30,899,684
        10.8
        S3W9
        32,041,201
        11.2

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        TAACGT
        141501.0
        1.1
        TAACTT
        124080.0
        0.9
        CAACGT
        101997.0
        0.8
        TAAGTT
        99824.0
        0.7
        TAAGGT
        90174.0
        0.7
        CAACTT
        85101.0
        0.6
        CCCCGC
        83246.0
        0.6
        TACCGT
        82927.0
        0.6
        CAAGTT
        65204.0
        0.5
        TACCTT
        63840.0
        0.5
        CACCGT
        63413.0
        0.5
        CAAGGT
        63401.0
        0.5
        CCCCCC
        60952.0
        0.5
        GTTCGG
        58974.0
        0.4
        TCACGT
        56831.0
        0.4
        CCACGT
        55053.0
        0.4
        TAACGG
        54872.0
        0.4
        TAAAGT
        53609.0
        0.4
        TCACTT
        53479.0
        0.4
        CAACGG
        51857.0
        0.4

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        8.0
        311,107,616
        285,651,261
        4.7
        5.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.