FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13515751
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN268380.198568322248612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA3350.022.13624228
AGTGTAG3350.021.4651329
TCTCGGT3750.021.0441137
GTAGATC3650.020.93194432
CACTCTT6200.020.3197332
GTGTAGA3800.020.1051630
TGTAGAT4250.017.44753331
TCGGAAG9500.017.2853163
ATCGGAA9450.017.1404552
AGTTAGG1205.1881325E-616.8726981
GATCGTA800.001717104516.8676855
GATCTCG5050.016.46479835
GATCGGA9850.016.4444561
TAGATCT4800.015.91733
CGGAAGA10850.015.1334294
TCGTGTA5200.014.69315915
CTCGGTG5850.014.6801338
GTGTAGG5850.013.83116517
AGATCTC6550.013.0367434
TCCGATT4000.012.9357351
Produced by FastQC (version 0.11.5)