FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32041201
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29850.013.0521412
GTACTCG73300.012.0723684
TACTCGA75750.011.6209665
AGTACTC80900.011.384593
GCGTGAC41750.010.2489131
GTAGAAC39600.09.59479511
GGAAAAT135200.08.9283011
AATCGAA56600.08.8702645
CTCTATA75900.08.812745
GTGACTC47600.08.7046943
GAATCGA46900.08.6417874
CACTCGT79000.08.51394435
CACGTAA44350.08.36864616
ACTCGTG81800.08.25203836
AAAATCC97900.08.2346093
CGCGTAT34200.08.21730610
GAATTGA76000.08.2067274
ACGTAAT45500.08.15862417
TCACTCG81000.08.136834
CTAGGTA29450.08.09776117
Produced by FastQC (version 0.11.5)