FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30899684
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27900.012.5117192
GTACTCG56750.010.5941084
GTAGAAC41100.010.066092511
AGTACTC66600.09.5354333
TACTCGA62950.09.4066565
GCGTGAC41050.09.2718241
AATCGAA52800.09.1254675
GGAAAAT123400.08.9975311
GAATCGA43400.08.6645444
CTCTATA81600.08.61061645
CACTCGT83800.08.58985735
GCGGAAA95500.08.4896681
ACTCGTG83550.08.45610236
TCACACG27100.08.3002215
TCACTCG85650.08.167603534
GTGACTC47750.08.0175383
GAATTGA79050.07.9473314
TCAGGTA59650.07.8420717
TAGAACA54400.07.81248812
GGGAAAT65150.07.70871541
Produced by FastQC (version 0.11.5)