FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32804928
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC46050.09.76485511
GTACTCG58800.09.2672564
CGTGACT29150.08.6529392
AGTACTC71200.08.2865973
GGAAAAT130750.08.2849931
TACTCGA66050.08.214815
AATCGAA64200.07.9956395
GAATCGA59900.07.7437894
AAAATCC98250.07.6324973
GGTAGAA61500.07.604202710
GCGGAAA72800.07.5173311
TAGAACA61850.07.48934812
GCGTGAC37300.07.4868941
TCAGGTA70550.07.33161647
GGGAAAT84050.07.1542211
CTCGAAT28700.07.1408241
AAATCCA120850.07.0616414
AAAAATC95150.06.9822822
AATTGAC52700.06.92082265
GGGGAAT66850.06.90623331
Produced by FastQC (version 0.11.5)