FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30897043
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC55650.011.47390611
TCAGGTA70350.010.2293367
GGTAGAA69650.09.29678910
CAGGTAG72950.09.1547018
TAGAACA71900.09.06929812
GGAAAAT160400.08.1157071
AGGTAGA85900.07.82599649
AAAATCC110450.07.7070233
GTACTCG40400.07.46871574
ATCAGGT124850.07.45854956
AGAGATC148300.07.45644762
GAATTGA95150.07.3836024
TATAGAA47050.07.37163731
AGATCAG138350.07.35667854
GGGGAAT68700.07.3105661
AAAAATC115950.07.2642782
AATCGAA62500.07.13260945
GGAGAAT117800.07.1312631
GAATCGA51850.06.99197054
GGAAGAT226800.06.96111
Produced by FastQC (version 0.11.5)