FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29606654
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT33600.015.2820382
GCGTGAC46300.012.3062561
GTACTCG65950.011.3695054
GTGACTC50500.011.0589063
AGTACTC74000.010.5596523
TACTCGA72600.010.2027785
GTAGAAC40550.010.20219311
GAATCGA41600.09.7970954
GGAAAAT120500.08.8962371
GCTCTAG65700.08.82919111
AAAATCC88800.08.7743513
AATCGAA52900.08.7673985
TGACTCT69800.08.5810084
GGGAAAT58800.08.5794041
GAATTGA70400.08.50787454
CTCGAAT24550.08.4396171
AGCCTAG24000.08.43668725
CTCTATA71500.08.03183445
CACTCGT73550.07.921413435
TAGAACA53550.07.852226712
Produced by FastQC (version 0.11.5)