FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43398487
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC79150.011.1068611
TCAGGTA97800.09.7496997
CGTGACT31850.09.0506822
TAGAACA101000.08.90547812
GGAAAAT212300.08.8151921
GGTAGAA104750.08.456840510
GTACTCG80650.08.3756754
CAGGTAG109550.08.354098
AATCGAA81100.08.1616455
GAATCGA67950.07.88661154
AAAATCC152750.07.76921273
AGGTAGA120250.07.51636659
AATTGAC69900.07.50464255
ACCTAGG35850.07.403819615
TACTCGA92250.07.3948185
AATTCAC66800.07.2462495
GAATTGA107850.07.2445644
AAATCCT159950.07.22164344
AGATCAG179350.07.20631844
GGGAAAT103700.06.92775771
Produced by FastQC (version 0.11.5)