FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32386272
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC52650.011.14546111
TCAGGTA71400.09.1654577
CTAATCG34000.09.0737723
GGAAAAT181550.08.8943781
TAATCGA39650.08.8022914
AATCGAG68100.08.6618425
TAGAACA69350.08.62450312
CAGGTAG75200.08.1331738
AAAATCC126000.08.0960633
GGTAGAA75050.08.02860410
GGGAAAT90800.07.7385821
GGAGAAT111600.07.58780771
GAATTGA88800.07.3788264
AGGTAGA85300.07.2746929
AAATCCT129900.07.24559934
GGTAAGT24550.07.239730417
AGAGATC143800.07.04753972
AATCCTT109600.07.02540975
CGTAACG24400.07.011625342
AATTGAC59200.06.997655
Produced by FastQC (version 0.11.5)