FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n02_S3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n02_S3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40067095
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT37550.013.0148392
GTACTCG83150.010.9944484
GCGTGAC54550.010.6103331
TACTCGA87600.010.0491055
AGTACTC95800.09.8962913
GAATCGA58100.09.1463014
GTAGAAC49250.08.9019811
CTCTATA86850.08.79057545
GGAAAAT175150.08.76929951
GTGACTC60150.08.72320653
AATCGAA70800.08.7130875
CACTCGT87900.08.57315435
ACTCGTG90650.08.33989236
GCGGAAA105700.08.330911
GAATTGA100450.07.93529224
TCACTCG94100.07.912392634
GGGAAAT84800.07.86115741
AAAATCC130950.07.68701743
AATTGAC64750.07.68435145
ACACGCG38550.07.52656466
Produced by FastQC (version 0.11.5)