FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32041201
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC69800.030.97488238
ACGTCTG75500.030.59241315
CCGTACG77000.029.27451733
CGTCTGA79200.029.16385716
ACCGTAC86000.026.57462332
CACCGTA86250.026.30811331
CTCGTAT57000.024.77337644
CACGTCT95800.024.41431414
TCGGAAG95750.024.100833
TCGTATG53600.023.94897845
ATCGGAA96300.023.9164712
TACGTAA98900.022.33670436
CACACGT104600.022.31622112
GTAATCT96150.022.252239
ACGTAAT99250.022.07673337
CAGTCAC106950.021.55252327
AGTCACC116600.020.13659728
ACACGTC121350.019.4772113
AATCTCG94850.019.37531341
TAATCTC112600.018.9613240
Produced by FastQC (version 0.11.5)