FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30899684
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG42850.029.9558933
AGTCACG49700.026.42669928
ACGTCTG52150.025.74513815
ACGTTTC52300.024.71308332
TTCGGAA51200.024.10291136
TTTCGGA52400.023.85176835
CGTCTGA57100.023.51389916
GTTTCGG54900.023.4629534
GTCACGT64050.020.40914529
CACGTTT68400.019.45096431
TCGGAAG70150.019.1086653
CACGTCT73100.018.85837414
ATCGGAA72850.018.3695772
TCGGAAT71650.016.97265837
CACACGT81800.016.9619112
CAGTCAC82300.016.3670327
CGGAATC74150.016.31104938
GGAATCT79450.014.429399539
ACACGTC96500.014.42482113
TCGTATG32100.013.12001145
Produced by FastQC (version 0.11.5)