FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32804928
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG50650.024.55399515
AGTCACG50100.024.28521728
CGTCTGA53300.023.29140516
CACGTGG57250.021.07655531
GTCACGT65700.018.38931529
TCGGAAG69850.018.0639483
CACGTCT71100.018.06047214
ATCGGAA70250.017.865072
GGCCTTA68350.017.56108936
GCCTTAT67700.017.33092537
ACGTGGC73800.016.5066132
CACACGT78850.016.3702612
TCGTATG38950.015.66966645
TCACGTG78850.015.3276530
CAGTCAC87800.014.41980227
ACACGTC94750.013.83696113
TATCTCG60600.013.75116741
CTTATCT82600.013.71531339
CTCGTAT47600.013.43754444
CCTTATC90550.013.03338638
Produced by FastQC (version 0.11.5)