FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30897043
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGC41300.010.18199327
AGTCACG33550.09.585690528
TTAGCGT40650.09.4043678
GTGAAAT88750.09.275551
CCGTAAT29550.09.2098981
GTTAGCG40850.09.1931777
ACGTCTG37200.09.12877515
TCGTATG33800.08.99536345
CGCGTAT14550.08.965861
GTCCTAT28150.08.96116543
TAGCGTC45850.08.77929
CGTGAAA37850.08.56746933
GGCGAAT28050.08.4996291
CCCGACT23050.08.48936751
CGTCTGA40400.08.40579816
CTCGTAT33800.08.39608144
ATTTAGC87150.08.1280136
AATCCCG40100.08.020315
AATTTAG85500.07.996635
GTCACGT40800.07.830460529
Produced by FastQC (version 0.11.5)