FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29606654
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG32500.017.50773628
ACGTCTG33850.017.27417215
CGTCTGA37200.015.65837816
ACGTCCG38950.014.68358532
TCGTATG30000.014.18814945
GTCCGCA40350.013.95229634
CTCGTAT33350.013.16863844
TCGGAAG46550.012.5623253
GTCACGT50650.011.46049329
CACGTCT53650.011.44364714
CCGCACA50300.011.23711836
ATCGGAA54200.010.7892282
TCACGTC56500.010.43630
TATAGGC53150.010.02759627
CAGTCAC62100.09.92226327
CACACGT61450.09.8442412
CGCACAT60900.09.46638937
GGCGAAT34850.09.2937281
GTCGGTT29950.09.0869651
TCCGCAC69200.08.91648535
Produced by FastQC (version 0.11.5)