FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43398487
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG101100.029.80790515
CGTCTGA106600.028.3548516
GTCCGCA108050.026.05202136
ATCGGAA128000.023.545762
TCGGAAG132350.022.924793
ACCCGTC129350.022.47384632
CACACGT136750.022.15080612
CACGTCT137500.022.12900514
CTCGTAT69200.021.67558944
TCGTATG66100.020.85159145
CGCATCT121600.020.09606239
CAGTCAC151050.020.03888927
ACACGTC158500.019.39521413
CCGTCCG158650.019.16235434
AGTCACC160600.019.12894428
TCCGCAT143100.019.02625737
TCACCCG157350.018.99452830
CCCGTCC159450.018.54352633
CGTCCGC169800.017.86426235
GATCGGA178450.017.1415751
Produced by FastQC (version 0.11.5)