FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32386272
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52550.022.4682115
CGTCTGA54200.021.66017216
ATCGGAA69000.016.9502092
TCGGAAG75100.015.9328153
CACACGT75800.015.63485212
CACGTCT80750.015.0391214
ACACGTC84000.014.4301213
GTCAGAA85200.013.63828336
CAGTCAC91200.013.04407327
TGTCAGA90700.012.93538435
GTCACAT91400.012.8003629
AGTCACA93200.012.49984228
GATCGGA99300.011.9822761
GTCTGAA109750.011.00424917
ATGTCAG109650.010.9668434
CACATGT115150.010.34167731
ACATGTC119600.010.03459732
CCAGTCA118650.010.00733926
TCCAGTC124050.09.89801925
TATGGGT42450.09.762012543
Produced by FastQC (version 0.11.5)