FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l08_n01_S3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l08_n01_S3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40067095
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG39500.018.86945734
TCCGTAT37950.018.8693737
CCGTATC40050.017.8818238
ACGTCTG43450.017.85737815
CGTATCT40950.016.99399639
CGTCTGA49250.015.61763616
TTCCGTA50300.014.59412836
GTTCCGT62650.012.11259935
TCGTATG36250.011.61726645
TCGGAAG67050.011.6066933
CACGTCT72750.011.31420314
ATCGGAA68150.011.2543322
TATAGGC76800.010.57079927
CTCGTAT43900.010.56786244
GTCACAG78950.09.888629
CACACGT82150.09.82762712
CAGTCAC86750.09.48797227
AGTCACA90150.09.18087428
GGCGAAT43350.09.0282661
TTATAGG88000.08.94431726
Produced by FastQC (version 0.11.5)