FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12907061
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT3400.029.84982737
GTAGATC4050.025.51451132
TAGATCT4150.024.899833
GAGTGTA3700.024.89635728
CACTCTT6350.023.7382032
AGTGTAG4050.022.74181429
GTGTAGG5000.020.2235317
GTGTAGA4500.019.96787630
TCGTGTA5000.019.3189215
TAGGGAA5250.018.40913620
CTCGGTG5800.018.3230738
GTAGGGA5300.018.23511119
TGTAGAT5300.017.80203631
TGTAGGG5850.017.2876918
GATCTCG6000.016.8517635
AGATCTC6350.016.2746834
AGTTAGG850.00256941715.8801791
TCGGTGG6850.015.18551739
CGTATAG900.003805034614.97119312
AGCGGTT3800.014.18257410
Produced by FastQC (version 0.11.5)