FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30144398
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27450.011.6533262
GTACTCG53650.010.2850314
GTAGAAC34000.09.8519211
GCGTGAC38350.09.69175151
AATCGAA53450.08.9326455
GAATCGA43200.08.9150054
AGTACTC64800.08.7946463
TACTCGA62250.08.7914165
AGCCTAG29950.08.71431825
GGAAAAT116800.08.6786741
CTCTATA76250.08.565989545
CACTCGT78850.08.30194435
GCGGAAA87550.08.181911
TCACTCG81250.07.862128734
GAATTGA67650.07.85694034
AATTGAC47050.07.8022515
GCATAGG36950.07.798618341
AAAATCC86800.07.7852823
ACTCGTG81500.07.730700536
AATTCAC44700.07.6582225
Produced by FastQC (version 0.11.5)