FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25448373
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45400.011.09199411
CTAATCG27550.010.9564473
TAATCGA32650.010.1405934
AATCGAG51550.09.8301835
TCAGGTA57750.09.4222437
GAATCGA37600.08.80559354
CAGGTAG59050.08.7186338
TAGAACA59450.08.47107112
GGAAAAT125250.08.3451571
AAAATCC81300.08.2845163
AATCGAA46550.08.2734035
GGTAGAA61300.08.25162410
GTACTCG33850.07.91803554
AATTGAC39150.07.76622155
GCTAATC49650.07.75882432
AGGTAGA68850.07.7058929
AAATCCT87900.07.66145854
CGTTAAC19250.07.47991421
ATCAGGT111450.07.4670556
AATCCTT78150.07.4064815
Produced by FastQC (version 0.11.5)