FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23813900
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23850.012.27913952
GCGTGAC34350.09.4433061
GTACTCG44600.09.2415744
GTGACTC37950.08.6663413
GTAGAAC32750.08.64933711
GAATCGA38600.08.460784
TACTCGA48250.08.1687025
AATCGAA45500.08.1179215
AGTACTC52350.08.0897643
CTCTATA56800.08.049437545
GGAAAAT92350.08.0494341
AGCCTAG20800.07.78818425
GCTCTAG42150.07.73381811
CACTCGT56650.07.6638335
GCGGAAA65000.07.65891461
GGAGAAT68600.07.58535861
ACTCGTG59250.07.51947936
AATCGAC28250.07.4941395
GGGAAAT55650.07.4480221
TCACTCG59600.07.32140834
Produced by FastQC (version 0.11.5)