FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24809537
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT21300.09.9414842
GTAGAAC32500.09.68389511
GGAAAAT97200.08.3196961
GGACTTA38000.08.2381684
GTACTCG39250.08.2053254
GACTTAT37450.08.0582065
GCGTGAC28550.07.968831
AGAACGG141300.07.8113363
TACTCGA43100.07.7333885
GAATTGA65200.07.59931564
GGGGAAT50200.07.5384991
CGTAGGT13200.07.498530435
GGGAAAT55050.07.48812441
AAGAACG171950.07.4675082
ACACGCG21400.07.46199856
AACGGCC95500.07.4519355
AATCGAA52700.07.43573575
AATTGAC42200.07.31128035
AAAATCC72150.07.2430763
GGTAGAA44850.07.21782510
Produced by FastQC (version 0.11.5)