FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29987229
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27750.011.9331762
GCGTGAC39050.09.171831
AATCGAA57400.08.7886685
GGAAAAT120550.08.7449751
GTACTCG51750.08.61724954
GAATCGA47800.08.528344
GTAGAAC40500.08.43728311
CTCTATA71100.07.887417345
TACTCGA56600.07.8783585
AGAACGG181250.07.54363063
CACTCGT71600.07.509056635
GACTTAT42900.07.4544915
AAAATCC90200.07.44182543
GCTCTAG53800.07.43792411
AGTACTC64750.07.4098533
GTGACTC48050.07.40684843
ATATCCA86200.07.394234
GAATTGA78950.07.3030054
GGACTTA40050.07.2543914
GCGGAAA88250.07.2235661
Produced by FastQC (version 0.11.5)