FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35112676
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC58950.010.48759911
AATCGAA66350.09.2325825
TCAGGTA75350.09.1910017
GTACTCG58450.08.862484
GGAAAAT176950.08.6563011
AAAATCC124250.08.5570113
GAATCGA58500.08.1619134
TAGAACA77350.08.13861112
CAGGTAG80750.08.1298368
GGTAGAA81350.07.986701510
TACTCGA68050.07.90979055
AATTGAC61000.07.90094765
CGTGACT26100.07.85403632
CTCGAAT33350.07.76740551
GAATTGA96600.07.647314
AATCCTT105500.07.55694065
GGGAAAT87950.07.4786121
AAATCCT126400.07.43020774
GGGGAAT76700.07.13649461
AGATCAG144950.07.11638164
Produced by FastQC (version 0.11.5)