FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29271796
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25050.011.1514182
GTACTCG48700.09.2959524
TACTCGA51500.08.7901655
GGAAAAT114350.08.7268231
GCGTGAC38600.08.5786441
AATCGAA53350.08.2742735
GAATCGA44850.08.2358464
AGTACTC59000.08.0559643
TATTCGC44150.07.53747757
ACACGCG25100.07.43750436
CTCTATA66250.07.411224445
AAAATCC83800.07.39223583
GGACTTA44500.07.38957834
GAATTGA73950.07.37061934
AAAAATC81150.07.35653832
TAGGCGG41950.07.344855331
GCATAGG42850.07.302732541
GGGAAAT66350.07.29938271
GAAAAAT135650.07.2734911
AGCCTAG29400.07.19349925
Produced by FastQC (version 0.11.5)