FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36090424
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC63700.011.046103511
CGTGACT24550.09.7267182
GGAAAAT172150.08.8716641
TCAGGTA84450.08.8127267
AATCGAA69200.08.7546715
TAGAACA81800.08.60241812
GGTAGAA86400.08.32607710
GGGAAAT91200.08.0767251
AAAATCC120100.08.0462293
GAATCGA53400.07.9713464
ATCTAAC26250.07.96831276
CAGGTAG90500.07.94927078
GTACTCG68000.07.9158974
GGTAAGT28050.07.779629717
GAATTGA91800.07.4338044
TACTCGA75150.07.4321825
CGAAATT48050.07.3138022
ACCTAGG33600.07.2958915
AAATCCT119950.07.2101144
ATCGAAC37350.07.16587266