FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n02_C3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n02_C3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40133139
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT38750.013.3711252
GCGTGAC53700.010.86451151
GTACTCG75550.09.6887774
GGAAAAT161300.09.0774461
TACTCGA80850.09.0255285
AATCGAA70250.08.8485155
GAATCGA60000.08.5586194
AGTACTC89250.08.55616953
GTGACTC64800.08.5516133
AATTGAC61550.08.1598915
CTCTATA91700.08.08062645
ACACGCG39650.08.0550366
GCTCTAG74650.08.01072711
GTAGAAC45850.07.99223711
GCGGAAA109950.07.88769631
GAATTGA95800.07.87588554
GGGAAAT83550.07.73784641
CACTCGT95100.07.711224635
AAAATCC115150.07.6489313
TTACGCG9850.07.53206548
Produced by FastQC (version 0.11.5)