FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12907061
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG3900.028.82765415
CGTCTGA3950.028.46274816
CACACGT4200.027.30147412
GAGACCG4150.024.4061531
ACACGTC4900.024.31924213
CACGTCT4950.023.62019714
CCGAGAC5100.020.29163729
ACCGATC5400.019.59613234
CAGTCAC4500.017.98685327
CGAGACC6150.017.92880830
CCAGTCA4600.017.59583526
CGCTTAC1053.092907E-517.15798843
GGAATGC6950.016.82236723
CCGATCT6200.016.7054335
AGGAATG6900.016.61841822
ATCGGAA10500.016.514052
AGACCGA6150.016.47366332
TCGGAAG10800.016.0553263
AGCGGTT7000.015.73849711
AGCACAC7600.015.38349210
Produced by FastQC (version 0.11.5)