FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30144398
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49000.027.2171415
CGTAATC46550.026.60910838
CGTCTGA52200.025.46270216
CCGTACG52000.024.55224833
TCGTATG42900.022.04913345
ACCGTAC60350.021.52767432
CACCGTA61400.020.86054431
CTCGTAT47750.020.84827244
TCGGAAG65050.020.5033653
ATCGGAA67500.019.5925662
CACGTCT70000.019.27622414
CACACGT76150.018.10285612
GTAATCT72450.017.28392839
CAGTCAC79150.016.76322727
TACGTAA81600.015.48168336
ACGTAAT81300.015.37272337
ACACGTC90850.015.22335213
AGTCACC90450.014.84316628
TAATCTC86350.014.58051440
AATCTCG83100.014.14792641
Produced by FastQC (version 0.11.5)