FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25448373
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG32800.031.51203233
AGTCACG39950.026.3439528
GTTTCGG39550.026.24820734
ACGTCTG42550.025.5283815
TTTCGGA43400.023.71345135
CGTCTGA46400.023.36180716
TTCGGAA43950.023.26315936
ACGTTTC45650.022.98629632
GTCACGT51800.020.45868329
ATCGGAA55950.019.5365432
TCGGAAG58600.018.6914443
TCGGAAT54750.018.55097237
CGGAATC59500.017.18474438
CACGTTT64750.016.41021231
CACGTCT68350.016.28659814
CACACGT66650.016.19525312
CTCGTAT35400.016.16054544
ACACGTC71950.015.69006313
TCGTATG35150.015.37753345
GGAATCT67950.014.7498239
Produced by FastQC (version 0.11.5)