FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23813900
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG39650.025.80786115
AGTCACG39350.025.54524828
CGTCTGA42850.023.93308816
CACGTGG44350.022.38441731
TCGTATG31450.021.05418245
GTCACGT50700.020.0593529
TCGGAAG52250.019.6713333
CACGTCT53650.019.40770114
CACACGT55150.019.08288612
ATCGGAA54100.019.0402262
ACGTGGC53450.018.87330432
GGCCTTA53800.018.71075436
GCCTTAT53400.018.3872137
CTCGTAT38650.018.1820744
CAGTCAC63300.016.30619627
TATCTCG53150.015.93405941
ACACGTC68950.015.78560413
CTTATCT63300.015.29976239
TCACGTG67950.015.10346830
CCTTATC65650.015.06019338
Produced by FastQC (version 0.11.5)