FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24809537
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG40900.025.01930215
AGTCACG41150.023.71833428
CGTCTGA43050.023.71759616
TCGTATG40800.022.7977945
CGTGAAA45050.021.54227433
ACGTGAA47050.020.76958332
CTCGTAT43300.020.49388744
CACGTGA49050.019.96309731
AACGATC45250.019.55913538
TGAAACG53000.018.6095335
GTCACGT53150.018.54151729
TCGGAAG57100.018.0797923
CACGTCT57500.018.0304714
GAAACGA56250.017.8147236
ATCGGAA59250.017.233932
AAACGAT57100.016.80039237
CACACGT63550.016.1365612
CAGTCAC68100.014.9263327
ACACGTC73550.014.49317413
CGGAAGA78700.013.5176864
Produced by FastQC (version 0.11.5)