FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29987229
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325400.10851286059142043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42950.023.51052915
AGTCACG42500.023.33508328
CGTCTGA46000.021.85393716
ACGTCCG48100.020.78467632
CTCGTAT45400.019.84399644
TCGTATG44850.019.53412645
GTCCGCA51500.019.15104734
GTCACGT58250.017.45909329
TCGGAAG59250.017.3474673
ATCGGAA60000.017.0556552
CCGCACA60750.016.49580236
CACGTCT64200.016.07849314
TCACGTC67650.015.03678130
CACACGT73050.014.37646112
CAGTCAC74250.014.20468927
CGCACAT72250.013.49616937
ACACGTC80800.013.1645913
TCCGCAC80900.012.693089535
CGTCCGC87800.011.74598333
CGGAAGA93750.011.6592634
Produced by FastQC (version 0.11.5)