FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35112676
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG58450.022.66279615
CGTCTGA61700.021.28689216
GTCCGCA64900.019.46590436
ATCGGAA74000.017.7799662
TCGGAAG83050.016.167453
ACCCGTC80700.015.98786332
CTCGTAT49450.015.89625544
CACACGT86150.015.47929512
CACGTCT87950.015.34198114
TCGTATG50100.015.10475545
CGCATCT81850.014.501142539
CCGTCCG93950.014.47654734
ACACGTC97200.014.18235813
CCCGTCC100100.013.18210233
CAGTCAC102200.013.02634527
TCCGCAT97000.012.9080737
TCACCCG104850.012.47086830
CGTCCGC113000.012.45519635
AGTCACC106200.012.45122228
GATCGGA109750.012.2137541
Produced by FastQC (version 0.11.5)