FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29271796
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG55650.026.83369615
CGTCTGA57400.025.97659516
TCGGAAG72450.020.612343
CACGTCT74150.020.4411614
ATCGGAA73600.020.3513892
CACACGT81050.018.92227412
GTCACAT80050.018.80399529
GTCAGAA78700.018.62833636
CAGTCAC84200.017.94730227
TGTCAGA84550.017.8185135
AGTCACA88550.017.06584728
ACACGTC91200.016.93968613
CGGAAGA100350.015.3073314
GATCGGA100550.015.03097251
TCGTATG34750.014.84270645
ATGTCAG103600.014.6499734
CCAGTCA107350.014.20265826
CTCGTAT39700.013.95725644
ACATGTC114500.013.43167832
AATCTCG70400.013.37377141
Produced by FastQC (version 0.11.5)