FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36090424
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG63100.027.87108434
TCCGTAT61650.027.46930537
CCGTATC62950.027.01099838
CGTATCT61400.026.77715339
ACGTCTG69900.025.83559215
CGTCTGA71650.025.0790616
TTCCGTA72700.023.2629536
CTCGTAT58500.021.7531644
TCGTATG56200.021.19979545
ATCGGAA85900.021.0247752
TCGGAAG89000.020.4440753
GTTCCGT84850.020.17036835
CACACGT95650.019.3492812
CACGTCT99000.018.62672214
TATCTCG79250.018.2459141
GTCACAG101800.017.59372529
ACACGTC109250.017.1259113
CAGTCAC110200.016.651727
TCACAGT111850.015.91640730
GATCGGA116050.015.7563251
Produced by FastQC (version 0.11.5)