FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l07_n01_C3W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l07_n01_C3W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40133139
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG73350.028.3601915
CGTCTGA77800.026.7382816
TCGTATG64150.025.03437645
CTCGTAT67700.024.15521244
TCGGAAG97300.021.5888333
ATCGGAA98250.021.196962
CACGTCT101250.021.05527114
CACAGTC103850.020.00846331
CACACGT107400.019.84892312
GTCACAG108150.019.2861929
CAGTCAC113600.018.448927
AGTCACA116100.017.95497328
AGTCAAC117650.017.64785234
AATCTCG108450.016.98956741
ACACGTC129100.016.96552313
TCACAGT126350.016.44135930
ACAGTCA133950.015.65113332
GATCGGA137150.015.3980221
GTCAACA136600.015.15108435
CGGAAGA145850.014.7569954
Produced by FastQC (version 0.11.5)