FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN677840.4860801612044439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT7700.026.8766922
AGTGTAG2950.023.61283529
GAGTGTA3000.023.2195428
TAGATCT3250.022.81663333
GTGTAGA3150.022.11177430
GTAGATC3400.021.14788832
TCTCGGT3400.020.56312237
TCCCTAC4750.019.3860979
ATCGGAA9450.018.8050292
TCGGAAG9500.018.7058533
TTCCCTA4700.018.1602548
CTCGGTG4750.017.60420238
TGTAGAT4150.017.32510431
GATCGGA10450.017.220081
GATCTCG4650.016.43053435
TACACGA4050.016.08634813
TCGGTGG4750.015.70309539
AGCGGTT5750.015.23351110
CGGAAGA11700.015.1868684
CGGGAAT900.00376368414.9958191
Produced by FastQC (version 0.11.5)