FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25387828
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24900.014.3796722
GTACTCG61700.012.115724
GTAGAAC33350.011.39236511
AGTACTC68750.011.2999863
TACTCGA67100.011.1733575
GCGTGAC36900.010.3131411
GGAAAAT107400.09.8123311
GTGACTC39600.09.6099923
GGGAAAT53500.09.1755761
AAAATCC76450.09.1309443
AATTGAC39900.09.0283645
GAATTGA56450.08.8948214
GAATCGA37800.08.8158914
GGTAGAA46950.08.61906110
ATTGACC41000.08.50239856
GCTCTAG48350.08.46248511
AAATCCT83350.08.3473614
AATCGAA45850.08.2986865
ATCTAAC12500.08.09647756
TAGAACA46650.08.09615712
Produced by FastQC (version 0.11.5)