FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25736957
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG32900.013.7577053
TAATCGA40450.012.5806654
GTAGAAC45900.011.95105711
AATCGAG60950.010.4539975
TAGAACA57400.09.55664512
GAATCGA37100.09.2860534
TCAGGTA64450.09.2444827
GCTAATC54300.09.20692
AATCGAA46000.08.9570065
GGAAAAT133700.08.8929841
GGTAGAA61750.08.847033510
CAGGTAG63800.08.8453548
AAAATCC91150.08.6715413
AGGTAGA64600.08.5958289
GAATTGA62850.08.132694
AATCGTA13500.08.0052915
AGCATAA143850.07.98233465
CATAAGC146450.07.8910257
AAATCCT98300.07.81114154
AATCCTT87500.07.69365645
Produced by FastQC (version 0.11.5)