FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29959625
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27300.012.8682862
GTAGAAC44700.010.36086511
GTACTCG55450.010.1111334
GCGTGAC39350.09.2134691
CTCTATA76000.08.65334445
TACTCGA64950.08.63157755
GGAAAAT116650.08.5904771
AGTACTC70350.08.5465033
CACTCGT78550.08.36204835
ACTCGTG79900.08.27892436
GAATCGA46850.08.1703624
CTCGAAT25700.08.1487661
GGTAGAA58250.08.14367910
GTGACTC48150.07.9972583
TCAGGTA59700.07.98404937
GGGAAAT71050.07.89182141
GCGGAAA95900.07.84277731
TAGAACA58400.07.737732412
TCACTCG83450.07.736268534
AATCGAA52700.07.7328215
Produced by FastQC (version 0.11.5)