FastQCFastQC Report
Sun 5 May 2019
CCLM1ACXX_l06_n02_B3W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCLM1ACXX_l06_n02_B3W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25865151
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22150.011.0817782
GTAGAAC39850.010.88793811
GTACTCG49800.010.1729634
TAGAACA49100.09.01985812
GGAAAAT112100.08.9593881
GAATCGA40600.08.87336354
AGTACTC60950.08.8668763
TCAGGTA54500.08.827977
TACTCGA56550.08.7986845
GCGTGAC31800.08.568551
GGTAGAA51500.08.5558810
AATCGAA46850.08.4098185
AAAATCC80150.08.1475593
CTCGAAT23900.07.91462761
CGAAAAT26100.07.6788921
GAATTGA63300.07.5765274
CAGGTAG61150.07.57388168
AGAACGG157600.07.5584463
ATCTAAC14550.07.41923146
AAATCCT87250.07.3548494
Produced by FastQC (version 0.11.5)